Gasdermins are pore-forming proteins, mediating a highly inflammatory immune response in mammals, often culminating in a regulated cell death, termed pyroptosis. Microorganisms – fungi, bacteria and archaea – also encode for members of the gasdermin family. Some fungal gasdermins (fGSDMs) have been shown to control organismal defense, specifically allorecognition cell death. Moreover, the majority of fGSDM genes are genomically clustered with protease-encoding genes. The latter encode proteases controlling the cytotoxic activity of the fGSDM through proteolysis, as described in mammals and bacteria. We aim to explore further the molecular mechanisms fGSDM cytotoxicity and decipher the biological roles of fGSDM-depended pathways.